Technomyrmex albipes Testing for Wolbachia

November 21, 2023

Sample information
Picture
Photos by: Ryan Schlatter
Location
Collection date 09/06/2023
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Small black ant, with lighter colored feet, living on a tree, walking in lines.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

I am doing 5 samples of the same species from the same colony.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

In row 1 lanes 3-7 were all arthropod PCR, with positive bands in lanes 3 and 4. The 5-7 lanes did not correctly extract DNA so there was no band. Positive Wolbachia bands were shown in row 2 lanes 3 and 4. Row 2 in 5-7 lanes there was no Wolbachia band. All the samples of the same species tested positive for Wolbachia when the DNA was correctly extracted with a positive arthropod PCR CO1 band.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGAATAATTGGATCTTCTATAAGAATAATTATTCGTATTGAATTAGGAACCTGCAATTCTCTAATTAATAATGATCAAATTTATAATTCTATTGTTACAGGTCACGCATTTATTATAATTTTTTTTATAGTCATACCATTTATAATTGGTGGATTTGGAAATTTTTTAATTCCTTTAATATTAGGAGCCCCAGATATAGCATACCCACGAATAAATAATATAAGATTTTGACTATTACCACCCTCTATTATTATACTAACTATTAGTAATTTAATTGGATCAGGAGTAGGTACAGGATGAACAGTATACCCCCCTTTATCATCAAATATTTACCATAATGGACCCTCAGTAGATTTAGCAATTTTTTCTCTTCATATTGCTGGAATATCCTCTATTATAGGGGCTATTAACTTTATCTCAACTATCTTAAACATACACCATAAAAATTTAACTTTAGATAAAATTCCTTTATTAGTCTGATCTATTTTAATCACTGCAATTCTCTTATTACTCTCCCTCCCCGTCTTAGCCGGAGCTATTACCATACTTCTTACAGATCGAAATATCAATACTTCATTCTTTGACCCATGCGGGGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be postive for Wolbachia.
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