|Captive / Cultivated?||Wild-caught|
|Group||Hawaii Community College|
Small black ant, with lighter colored feet, living on a tree, walking in lines.
|Putative identification||Arthropoda Hexapoda Insecta Hymenoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Edvotek Gel Electrophoresis|
|DNA stain||SYBR Safe|
I am doing 5 samples of the same species from the same colony.
|Explanation of confidence level||
In row 1 lanes 3-7 were all arthropod PCR, with positive bands in lanes 3 and 4. The 5-7 lanes did not correctly extract DNA so there was no band. Positive Wolbachia bands were shown in row 2 lanes 3 and 4. Row 2 in 5-7 lanes there was no Wolbachia band. All the samples of the same species tested positive for Wolbachia when the DNA was correctly extracted with a positive arthropod PCR CO1 band.
|Wolbachia 16S sequence||
|Arthropod COI sequence|| Download FASTA
GGAATAATTGGATCTTCTATAAGAATAATTATTCGTATTGAATTAGGAACCTGCAATTCTCTAATTAATAATGATCAAATTTATAATTCTATTGTTACAGGTCACGCATTTATTATAATTTTTTTTATAGTCATACCATTTATAATTGGTGGATTTGGAAATTTTTTAATTCCTTTAATATTAGGAGCCCCAGATATAGCATACCCACGAATAAATAATATAAGATTTTGACTATTACCACCCTCTATTATTATACTAACTATTAGTAATTTAATTGGATCAGGAGTAGGTACAGGATGAACAGTATACCCCCCTTTATCATCAAATATTTACCATAATGGACCCTCAGTAGATTTAGCAATTTTTTCTCTTCATATTGCTGGAATATCCTCTATTATAGGGGCTATTAACTTTATCTCAACTATCTTAAACATACACCATAAAAATTTAACTTTAGATAAAATTCCTTTATTAGTCTGATCTATTTTAATCACTGCAATTCTCTTATTACTCTCCCTCCCCGTCTTAGCCGGAGCTATTACCATACTTCTTACAGATCGAAATATCAATACTTCATTCTTTGACCCATGCGGGGGABLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Hymenoptera was found to be postive for Wolbachia.|