Ochetellus Glaber (Wolbachia Negative)

Sample information

Picture
Photos by: Thomas A.
Location
Collection date 09/06/2023
Captive / Cultivated? Wild-caught
Group Hawaii Community College
Observations

Based on my observations, The black household Ant is an black and brown ant that feeds off of natural sugars and is present in large numbers when feeding off of natural sweet liquids. This Ant can be distinguished by its large bulbous head (Bull like) with small antenna and long yellowish brown legs. The color of the ant is black for the most part with large pincers in its cranial cavity. The thorax of this ant is rather fair and larger by the cranium and smaller near the abdomen. The abdomen is rather big and is slightly larger than its head. The ant belongs in the Hymenoptera order in the Ochetellus Genus. The scientific name of this ant species is Ochetellus Glaber.  I captured this specimen in my upstairs attic in Ocean View by adding natural honey unto a wooden bowl and naturally allowing the ants to crawl inside of the bowl. (Same observations as the Wolbahcia postive ant sample).

Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Table 1: Gel Loading Key for CO1 Gel (Well/lane for top gel)

Lane # Sample:
1 DNA ladder
2 TNA Ant 1
3 TNA Ant  2
4 W(+) Fruit fly control
5 W(-) Fruit fly control
6 (+) DNA control
7 Water 

Table 2: Gel Loading Key for 16S rRNA gel (Well/lane for bottom gel)

Lane # Sample:
1 DNA ladder
2 TNA Ant 1
3 TNA Ant  2
4 W(+) Fruit fly control
5 W(-) Fruit fly control
6 (+) DNA control
7 Water 

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Well #3 for the 16S gel had no band and the Wolbachia negative controls showed no band in the gel as well which confirms the negative result. The positive controls worked as well indicating a functioning result.

Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Hymenoptera was found to be negative for Wolbachia.
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