Sample information
Picture	Photos by: Lily P
Location
Collection date	11/09/2023
Captive / Cultivated?	Wild-caught
Group	John Overton High School
Observations	The spider was found under a cluster of rocks near a stream.
Putative identification	Arthropoda Arachnida Araneae Araneidae
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary