Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | I found the in a rotten log. There were pockets in the log and it was very hollow. The arthropod was found on top of the log walking around. This log was also close to a very large tree and off the trail not near any water. |
| Putative identification | Arthropoda Chelicerata Arachnida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | body without wings |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | After doing the gel Electrophoresis it was obvious that my sample contained the Arthropod Barcoding Gene (CO1) but did not contain the Wolbachia Specific Gene (16S rRNA). The higher bar around 708bp appeared with a bright florescent color. There was no bar at all around 438bp which is where a positive Wolbachia Gene would be located. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arachnida was found to be negative for Wolbachia. |
Water Boatman