Daddy long legs collection

November 29, 2023

Sample information
Picture
Photos by: Gavin B.
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

I found the in a rotten log. There were pockets in the log and it was very hollow. The arthropod was found on top of the log walking around. This log was also close to a very large tree and off the trail not near any water.
Putative identification Arthropoda Chelicerata Arachnida

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

After doing the gel Electrophoresis it was obvious that my sample contained the Arthropod Barcoding Gene (CO1) but did not contain the Wolbachia Specific Gene (16S rRNA). The higher bar around 708bp appeared with a bright florescent color. There was no bar at all around 438bp which is where a positive Wolbachia Gene would be located.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be negative for Wolbachia.
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