Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/09/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Gymnasium Immensee |
| Observations | Weather: High humidity, no rain, cloudy, wet Location: Underneath the ceiling of a bus stop |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Muscidae Musca Musca domestica |
Methods |
|
| Extraction kit | BABEC |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | On the Gel image, the respective lane is the fourth from the left (we were unable to extract DNA, so there’s no colour). |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We worked through the different steps carefully. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Musca domestica was found to be negative for Wolbachia. |
Water Boatman