Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/14/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We did not see clear results with the gel, so we did not send the sample for sanger sequencing; as a result, we cannot definitely state that the sample is wolbachia positive or not wolbachia positive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
ATTATTCGATTAGAATTAGGATCCCCTGATTCACTAATTCTTAATGATCAAACCTTCAATACTATCGTTACAAGCCATGCTTTTATTATAATCTTTTTCATAGTTATACCTTTTATAATTGGAGGATTTGGTAACTTCTTAGTACCCTTAATACTTGGGTCTCCTGATATAGCCTATCCACGCATAAATAACATAAGATTCTGATTACTACCCCCTTC AATTTCTCTATTAATCTTAAGAAATTTTATTAATGAAGGATCAGGAACAGGTTGAACTATTTATCCCCCCCTATCATCTAATACCTTTCATAGTGGTCCCTCTATTGATTTAACTATTTTTTCCCTTCATATTGCCGGAATATCATCAATTCTAGGGGCAATCAATTTTATTTCAACAATTTTAAACATACATAATTCTAATATTACCTTAGATAAAA TTCCCCTATTAGTATGATCTATTCTTATCACTGCTATTCTCCTCCTCCTCTCCCTTCCTATTCTTGCAGGAGCTATTACAATACTACTTACAGATCGAAATCTCAATACTTCATTTTTTGATCCCTCAGGAGGAGGAGACCCTATTCTATATCAACACTTATTTTGATTTTTTGGTCACCCTGAAAGTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Metriocnemus fuscipes YC1
Trachelipus ratzeburgii YC2
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1