Camponotus castaneus

January 8, 2024

Sample information
Picture
Photos by: Saanvi V
Location
Collection date 11/14/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

We did not see clear results with the gel, so we did not send the sample for sanger sequencing; as a result, we cannot definitely state that the sample is wolbachia positive or not wolbachia positive.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
ATTATTCGATTAGAATTAGGATCCCCTGATTCACTAATTCTTAATGATCAAACCTTCAATACTATCGTTACAAGCCATGCTTTTATTATAATCTTTTTCATAGTTATACCTTTTATAATTGGAGGATTTGGTAACTTCTTAGTACCCTTAATACTTGGGTCTCCTGATATAGCCTATCCACGCATAAATAACATAAGATTCTGATTACTACCCCCTTC AATTTCTCTATTAATCTTAAGAAATTTTATTAATGAAGGATCAGGAACAGGTTGAACTATTTATCCCCCCCTATCATCTAATACCTTTCATAGTGGTCCCTCTATTGATTTAACTATTTTTTCCCTTCATATTGCCGGAATATCATCAATTCTAGGGGCAATCAATTTTATTTCAACAATTTTAAACATACATAATTCTAATATTACCTTAGATAAAA TTCCCCTATTAGTATGATCTATTCTTATCACTGCTATTCTCCTCCTCCTCTCCCTTCCTATTCTTGCAGGAGCTATTACAATACTACTTACAGATCGAAATCTCAATACTTCATTTTTTGATCCCTCAGGAGGAGGAGACCCTATTCTATATCAACACTTATTTTGATTTTTTGGTCACCCTGAAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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