Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | |
| Group | Walton High School |
| Observations | Hard shelled, with orange tics on every column. Curled from the head when disturbed and after euthanization. |
| Putative identification | Arthropoda Myriapoda Diplopoda Polydesmida Euryuridae Euryurus Euryurus leachii |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We tested the Euryurus leachii for Arthropod Barcoding gene CO1 and Wolbachia specific gene 16sRNA. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | The results of the Euryurus millipede are not able to be compared because they are smeared. My group did not send our samples to be sequenced because there were errors in the gel electrophoresis and PCR making our results not trustworthy. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Metriocnemus fuscipes YC1
Trachelipus ratzeburgii YC2
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1