Sample information |
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Picture |
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Location | |
Collection date | 10/17/2023 |
Captive / Cultivated? | Wild-caught |
Group | College of Eastern Idaho |
Observations | |
Putative identification | Arthropoda |
Methods |
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Extraction kit | Edwards Buffer |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | In the gel image attached to this sample, there is a positive band that is very strong and solid as the control positive one thus, making it very obvious that our #1 AN sample is Wolbachia negative. |
Wolbachia 16S sequence |
>AN_SAMPLE6L_CO1 GGATATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
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Arthropod COI sequence |
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Summary | The Arthropoda was found to be negative for Wolbachia. |