Sample information |
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Picture |
|
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Location | |
Collection date | 08/01/2023 |
Captive / Cultivated? | Wild-caught |
Group | Stevens Point Area Senior High |
Observations | Male(Hairy Antenna) A. Aedes(White and Black along the legs) Spots along wings |
Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Aedes |
Methods |
|
Extraction kit | Edwards Buffer |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TBE |
DNA stain | GelRed |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Considering we had absolutely no bands, I am quite positive |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Aedes was found to be negative for Wolbachia. |