Melanoplus spretus

April 26, 2024

Sample information
Picture
Photos by: Julianna B
Location
Collection date 09/05/2023
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Sample collected by Fall 2023 Students.
Putative identification Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Low E-Value and high percent identity.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
Summary The Melanoplus was found to be negative for Wolbachia.
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