Sample information
Picture	Photos by: Julianna B
Location
Collection date	09/05/2023
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Sample collected by Fall 2023 Students.
Putative identification	Arthropoda Hexapoda Insecta Orthoptera Acrididae Melanoplus
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Low E-Value and high percent identity.
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA
Summary	The Melanoplus was found to be negative for Wolbachia.