Brown Recluse-1-4

September 11, 2023

Sample information
Picture
Photos by: Aditya N, Mukundh M, Sam P, Ajay M
Location
Collection date
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneae was found to be negative for Wolbachia.
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