Ant 1-4 Gold

September 11, 2023

Sample information
Picture
Photos by: Kenneth Xu
Location
Collection date 09/26/2020
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations

We used a trap to catch the organism; took place during the fall. Area was grassy but lacked other vegetation
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The experiment was done by the scientists at Vanderbilt, so the DNA extraction should have no faults.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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