Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Scientists in Residence |
| Observations | The Asian Lady Beetle was alive when caught. It was found crawling near the windowsill. The insect looked like a ladybug but it was later revealed to be an Asian Lady Beetle. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | My sample of the Asian Lady Beetle was placed in the second column in the gel. This column, as seen in the gel image above, had no band, indicating a negative result of Wolbachia. The positive control in column six worked and produced a positive band, this makes me feel confident in my conclusion. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGAC CAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTT TGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTT TACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCT CTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAAT TTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTG TATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACT GACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACATTTATTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Harmonia axyridis was found to be negative for Wolbachia. |
Water Boatman