Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/24/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Scientists in Residence |
| Observations | Found in spider web in windowsill. Already dead when caught. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Polleniidae Pollenia |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My gel test results showed that the cluster fly was positive for wolbachia based on the distance that the band traveled. Others who tested cluster flies for wolbachia also found the same results. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
NNNTNNNTTNNNNNNNNNANCTGGAATTATNGGTCGATTATTATCATTGATTATTCGCACTCAGCNAATGCACATTGNTA TACTTATCAATNACTATCAATTGTATAACTTAATGGTTACCGGGCATGCTTTGATAATGNTGTTTTTTATGATAATGCCA GCCCTCATGGGAGGATTTGGTAACTGGTTTGTACCTCTCATGATTGGCGCACCAAATATGGCGTTTCCTCATATGAATAA TTTGAGTTTCTGGTTATTACCGTCATCTTTTATTTTGCTCATTCTCTCANTGTTTATTGAAGAAGGTCCAGGTACAGGTT GGACTTTATATCCCCCTTTANCACACGTAATGTCCCATCCAAGTGCANGAGTTGACATTGCTATACTTGCACTTCATGTT GCTGGTATGTCNTCAATTGTTGGGGCNNNTTTTTTTATTCTTACTATTTTTAACATGCGCACAAAAGGANTGTCATTAAC TANCATGCCTCTGTTTGTTTGGTCTGTCTTGCNAACATCATTTATTTTGATTGTTGCCNTACCNNTCCNNGCCNGTGCTA TAACTATNCTTCNNACNGANTNNAAATANNTCANTTTNTNATNTGANCNTGCAGGTGGTGGNNACNCTGTGTNANTTCTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
AGTAGGAACCTCTCTAAGAATTTTAATTCGAGCAGAACTAGGACATCCTGGAGCATTAATTGGCGATGATCAAATTTATA ATGTAATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGA CTTGTCCCTTTAATACTAGGAGCACCTGATATAGCATTTCCTCGAATGAATAATATAAGTTTTTGACTTTTACCTCCTGC ACTTACACTACTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCTCCTCTATCTTCTA ATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATCTTTTCCCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCA GTTAATTTTATTACAACTGTTATTAATATGCGATCTACAGGAATTACATTTGACCGAATACCTTTATTTGTTTGATCTGT AGTAATTACAGCATTATTACTCCTTTTGTCCTTACCTGTATTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATT TAAATACTTCATTTTTTGACCCTGCA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Pollenia was found to be postive for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)