Cluster Fly

April 2, 2024

Sample information
Picture
Location
Collection date 03/24/2024
Captive / Cultivated? Wild-caught
Group Scientists in Residence
Observations

Found in spider web in windowsill. Already dead when caught.
Putative identification Arthropoda Hexapoda Insecta Diptera Polleniidae Pollenia

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My gel test results showed that the cluster fly was positive for wolbachia based on the distance that the band traveled. Others who tested cluster flies for wolbachia also found the same results.
Wolbachia 16S sequence Download FASTA    Download AB1
NNNTNNNTTNNNNNNNNNANCTGGAATTATNGGTCGATTATTATCATTGATTATTCGCACTCAGCNAATGCACATTGNTA TACTTATCAATNACTATCAATTGTATAACTTAATGGTTACCGGGCATGCTTTGATAATGNTGTTTTTTATGATAATGCCA GCCCTCATGGGAGGATTTGGTAACTGGTTTGTACCTCTCATGATTGGCGCACCAAATATGGCGTTTCCTCATATGAATAA TTTGAGTTTCTGGTTATTACCGTCATCTTTTATTTTGCTCATTCTCTCANTGTTTATTGAAGAAGGTCCAGGTACAGGTT GGACTTTATATCCCCCTTTANCACACGTAATGTCCCATCCAAGTGCANGAGTTGACATTGCTATACTTGCACTTCATGTT GCTGGTATGTCNTCAATTGTTGGGGCNNNTTTTTTTATTCTTACTATTTTTAACATGCGCACAAAAGGANTGTCATTAAC TANCATGCCTCTGTTTGTTTGGTCTGTCTTGCNAACATCATTTATTTTGATTGTTGCCNTACCNNTCCNNGCCNGTGCTA TAACTATNCTTCNNACNGANTNNAAATANNTCANTTTNTNATNTGANCNTGCAGGTGGTGGNNACNCTGTGTNANTTCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGTAGGAACCTCTCTAAGAATTTTAATTCGAGCAGAACTAGGACATCCTGGAGCATTAATTGGCGATGATCAAATTTATA ATGTAATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGA CTTGTCCCTTTAATACTAGGAGCACCTGATATAGCATTTCCTCGAATGAATAATATAAGTTTTTGACTTTTACCTCCTGC ACTTACACTACTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCTCCTCTATCTTCTA ATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATCTTTTCCCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCA GTTAATTTTATTACAACTGTTATTAATATGCGATCTACAGGAATTACATTTGACCGAATACCTTTATTTGTTTGATCTGT AGTAATTACAGCATTATTACTCCTTTTGTCCTTACCTGTATTAGCAGGAGCTATTACTATATTATTAACAGATCGAAATT TAAATACTTCATTTTTTGACCCTGCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Pollenia was found to be postive for Wolbachia.
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