Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/24/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | River Valley Community College |
| Observations | Arthropod appeared slow moving during capture. |
| Putative identification | Arthropoda Insecta Diptera Muscidae Musca Musca domestica |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | One positive and one negative control worked as expected. Any lack in confidence is attributed to a failed positive arthropod control and no positive result for the DNA control. It is expected that this is a result of a pipetting error when running the DNA extraction and isolation. Aside from this, the PCR ran as expected and the results were positive for both CO1 and 16S rRNA for Wolbachia. |
| Wolbachia 16S sequence | Download AB1
GGGGGGGGTTGGCGGATTCACAGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAAGCTAACC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence | Download AB1
CCATTTGGTATTTTATTTTTGGAGCATGAGCAGGAATAGTAGGAACTTCTTTAAGAATTTTAATTCGAGCAGAACTAGGACATCCTGGAGCACTAATTGGAGATGACCAGATCTATAATGTAATTGTAACAGCACATGCTTTTGTTATAATTTTCTTTATAGTAATACCAATTATAATTGGTGGGTTTGGAAATTGATTAGTTCCTTTAATACTAGGAGCTCCTGATATAGCATTTCCTCGAATAAATAATATAAGCTTTTGACTTTTACCTCCTGCTCTTACGCTATTGTTAATAAGCAGTATAGTGGAAAACGGAGCTGGAACAGGCTGAACTGTTTACCCACCACTATCTTCTAACATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTTTTCACTTCATCTAGCAGGAATTTCTTCAATTTTAGGAGCAGTAAATTTTATTACTACTGTAATTAATATGCGATCTACAGGTATTACTTTTGATCGAATACCCTTATTTGTTTGATCAGTAGTAATTACAGCTTTATTACTTCTTTTATCACTTCCTGTACTAGCAGGAGCAATTACAATATTATTAACAGATCGAAACTTAAATACTTCATTCTTTGACCCTGCGGGAGGAGGAGATCCAGTTCTATACCAACATTTATTTTGATTTTTTGGTCACCTGGAAAAGTTAAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Musca domestica was found to be postive for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica