Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/24/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Scientists in Residence |
| Observations | The fly was sitting on a carpet outside in the morning. It looked similar to a mosquito with long legs and a tail. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Mycetophilidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The Wolbachia band was absent. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Mycetophilidae was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)