Fungus Gnat

Sample information

Collection date 03/24/2024
Captive / Cultivated? Wild-caught
Group Scientists in Residence

The fly was sitting on a carpet outside in the morning. It looked similar to a mosquito with long legs and a tail.

Putative identification Arthropoda Hexapoda Insecta Diptera Mycetophilidae


Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level High
Explanation of confidence level

The Wolbachia band was absent.

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Mycetophilidae was found to be negative for Wolbachia.
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