Tachypodoiulus niger IM1

May 2, 2024

Sample information
Picture
Photos by: Iris M.
Location
Collection date 03/07/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

 

The Tachypodoiulus niger was found under the deadwood.
Putative identification Arthropoda Myriapoda Diplopoda Julida Julidae Tachypodoiulus Tachypodoiulus niger

Methods
Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

 

High=>Bacause the cytochrome oxidase I are present
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TAAGCATACTAATCCGAATAGAACTCTCTTACCCCGGAAGATTAATCGGAGATGACCAAATCTATAATGTTATCGTAACTGCTCATGCTTTTGWAATAATCTTTTTTATAGTTATACCTATTATAATTGGAGGGTTTGGAAACTGGCTTGTTCCTTTAATGTTAGGGGCTCCTGATATGGCTTTCCCTCGACTTAATAATATAAGCTTTTGACTTTTACCCCCTTCTTTATTTCTTTTATTAATATCCTCTGCGGTTGAAAAAGGAGCAGGAACAGGATGAACAGTCTATCCCCCTTTAGCAAATAATGTATCTCACTCAGGGCCCTCAGTAGATATAGCAATCTTTTCCCTTCATTTAGCAGGGGCTTCGTCAATCTTAGGAGCTATTAATTTTATTACAACTATTATTAACATGCGCTCAATAGGAATAGGATTCGAACATTTGCCTTTGTTTGTTTGAGCTGTAAAAGTAACAGCTATTTTACTTCTTTTATCTTTGCCTGTTTTGGCTGGAGCTATTACAATACTCTTAACAGATCGTAATTTTAATACCTCTTTCTTTGATCCTTCTGGCGGGGGAGATCCTATCTTATACCAACATCTTTTTTGATTTTTGGTCACTGKAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tachypodoiulus niger was found to be negative for Wolbachia.
Report Inappropriate Post