Sample information
Picture
Location
Collection date
Captive / Cultivated?	Wild-caught
Group	Acton-Boxborough Regional High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Hemiptera
Methods
Extraction kit	Puregene (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hemiptera was found to be postive for Wolbachia.