Sample information
Picture
Location
Collection date	10/02/2020
Captive / Cultivated?	Wild-caught
Group	Acton-Boxborough Regional High School
Observations	The woods near leary
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Geotrupidae Geotrupes Geotrupes splendidus
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Geotrupes splendidus was found to be negative for Wolbachia.