Polydesmus angustus RP2

May 2, 2024

Sample information
Picture
Photos by: Ramona P.
Location
Collection date 07/03/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

under the bark of a fir tree
Putative identification Arthropoda Myriapoda Diplopoda Polydesmida Polydesmidae Polydesmus Polydesmus angustus

Methods
Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
ATAAGAGGTTTTATTCGTTTGGAGTTGGGTGTTCCTGGAAGWWTCATAGGGGATGATCATATTTTTAATGTAGTTGTTACTGCTCATGCTTTTGTTATAATTTTTTTTATGGTTATACCAATTATAATTGGTGGTTTTGGAAATTGATTAGTTCCTATTATAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Polydesmus angustus was found to be negative for Wolbachia.
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