Armadillidium pulchellum IM2

May 2, 2024

Sample information
Picture
Photos by: Iris M.
Location
Collection date 03/07/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Under the bark of a red fir
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium pulchellum

Methods
Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

High=> Because cytochrome oxidase I and Wolbachia are present
Wolbachia 16S sequence Download FASTA    Download AB1
TGAGATGTTGGSTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGYGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCCTTCGAAGCTAGGAGCTTCGTCAATTTTAGGAGCTGTAAATTTTATTACTACTATAATTAATATACGGGCGGCTGGAATCACAATAGATCGAGTCTCTCTATTTGTTTGGTCTGKACTCATTACGGCAGTATTACTTCTTTTATCGTTACCTGTTTTAGCAGGGGCTATCACTATGCTATTAACAGATCGAAATTTGAATACTTCTTTTTTTGACCCGAGAGTAGTAGGAGACCCTATTCTTTATCAGCATTTGTTTTGATTTTTTGGTCACCTGAAAG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TCGGACAGAATTAGGACAGCCTGGAAGATTAATTAGGGATGATCAAATCTATAATGTAATTGTAACAGCGCATGCATTTGTTATAATTTTTTTCATAGTAATACCTATTATAATTGGGGGGTTTGGAAATTGATTAATTCCTTTAATATTAGGTGCTCCTGATATAGCTTTCCCGCGTTTAAATAACATAAGGTTCTGACTATTACCCCCTTCTTTAATTTTATTATTAAGTAGTGGTTTAGTTGAAAGGGGGGTAGGGACAGGATGAACCGTTTATCCTCCTCTAGCGTCTAGAATCGCTCATAGAGGAGCGTCTGTCGATTTAGGGATTTTTTCTCTACACCTAGCAGGAGCTTCGTCAATTTTAGGAGCTGTAAATTTTATTACTACTATAATTAATATACGGGCGGCTGGAATCACAATAGATCGAGTCTCTCTATTTGTTTGGTCTGTACTCATTACGGCAGTATTACTTCTTTTATCGTTACCTGTTTTAGCAGGGGCTATCACTATGCTATTAACAGATCGAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium pulchellum was found to be postive for Wolbachia.
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