Cylindroiulus caeruleocinctus NP2

May 2, 2024

Sample information
Picture
Photos by: Niclas P.
Location
Collection date 03/07/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

On the ground under a rotten branch covered with moss, wet environment
Putative identification Arthropoda Myriapoda Diplopoda Julida Julidae Cylindroiulus Cylindroiulus caeruleocinctus

Methods
Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

NP2: The stripes are very clear for the Wolbachia 16S rDNA and mitochondrial Cytochromoxidase. This means the process of the experiment worked well.
Wolbachia 16S sequence Download FASTA    Download AB1
ATTTCTAATAATTTTTTTTATAGTTATGCCTTTTTTAATTGGKGGWTTTGGRAATTATTTAATTCCTCTAAWATTAGGAYCACCAGAKATAGCATTTCCTCGAAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGACATCCTGGAATATGAATTAATAATGATCAAATTTATAATTCTTTAGTTACAAGYCATGCATTTCTAATAATTTTTTTTATAGTTATGCCTTTTTTAATTGGTGGWTTTGGGAATTATTTAATTCCTCTAATATYAGGAYCACCAGATATAGCATTTCCTCGAATAAATAATATWAGATTTTGAATTCTACCTCCTTCATTAATAATATTATTATTAAGAAATTTATTTACACCTAAKGTAGGAACAGGTGAACTGTATATCCTCCATTATCATCATATTTATTTCATTCATCACCTYCTGTWGACATTGCAATTTTTTCACTTCATATAACTGGAATTYCTTCAATTATTGGTTCATTAAATTTTATTGTTACTATWATAATAATAMAAAATTATTCATTAAGWTATGATCAAATYAWTTTATTYTCATGAKCAGTATGCATWACTGTAATWYTATTAATATTATCTYTACCTGTTTTAGCTGGAGCAMTTACAATATTACTTTTTGATCGAAATTTTAATACATCTTTTWTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Cylindroiulus caeruleocinctus was found to be postive for Wolbachia.
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