Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/07/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | On the ground under a rotten branch covered with moss, wet environment |
| Putative identification | Arthropoda Myriapoda Diplopoda Julida Julidae Cylindroiulus Cylindroiulus caeruleocinctus |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | NP2: The stripes are very clear for the Wolbachia 16S rDNA and mitochondrial Cytochromoxidase. This means the process of the experiment worked well. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
ATTTCTAATAATTTTTTTTATAGTTATGCCTTTTTTAATTGGKGGWTTTGGRAATTATTTAATTCCTCTAAWATTAGGAYCACCAGAKATAGCATTTCCTCGAAT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
AGACATCCTGGAATATGAATTAATAATGATCAAATTTATAATTCTTTAGTTACAAGYCATGCATTTCTAATAATTTTTTTTATAGTTATGCCTTTTTTAATTGGTGGWTTTGGGAATTATTTAATTCCTCTAATATYAGGAYCACCAGATATAGCATTTCCTCGAATAAATAATATWAGATTTTGAATTCTACCTCCTTCATTAATAATATTATTATTAAGAAATTTATTTACACCTAAKGTAGGAACAGGTGAACTGTATATCCTCCATTATCATCATATTTATTTCATTCATCACCTYCTGTWGACATTGCAATTTTTTCACTTCATATAACTGGAATTYCTTCAATTATTGGTTCATTAAATTTTATTGTTACTATWATAATAATAMAAAATTATTCATTAAGWTATGATCAAATYAWTTTATTYTCATGAKCAGTATGCATWACTGTAATWYTATTAATATTATCTYTACCTGTTTTAGCTGGAGCAMTTACAATATTACTTTTTGATCGAAATTTTAATACATCTTTTWTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Cylindroiulus caeruleocinctus was found to be postive for Wolbachia. |
Water Boatman