Acheta domesticus

April 7, 2024

Sample information
Picture
Photos by: Alex W
Location
Collection date 05/30/2024
Captive / Cultivated? Captive / Cultivated
Group River Valley Community College
Observations
Putative identification Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Lane 1: ladder

Lane 2: Sample 1 AW01

Lane 3: Sample 2 AW01

Lane 4: Sample 1 RD01

Lane 5: Sample 2 RD02

Lane 6: Wolbachia DNA

Lane 7: Water

Lane 8: (+) Control

Lane 9: (-) Control

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No banding at ~450bp on gel for 16s rRNA gene. All controls show that correct DNA was amplified for both arthropod CO1 and Wolbachia 16s rRNA genes.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
GCAAATTAACTTCATTTTCGGAGCTTGAGCTGGATAGTAGGTACCTCTTTAAGTATCTTAATTCGAACGGAACTAGGACAACCAGGTTATTTAATTGGAGATGATCAAACATATAATGTTATCGTAACTGCACATGCATTTGTCATAATTTTTTTCATGGTTATACCAATTATAATTGGTGGATTCGGAAATTGATTAGTACCCCTAATATTAGGTGCACCCGATATAGCCTTTCCTCGAATAAACAATATAAGATTTTGACTTTTACCACCCTCACTAACCCTTTTATTAACCAGAAGAATAGTCGAAAATGGTGCAGGGACAGGATGAACAGTTTATCCACCTTTATCAACAGGAATCGCCCACGCCGGAGCATCTGTTGATTTAGCCATTTTTTCATTACACTTAGCTGGAATTTCATCAATTCTGGGAGCCGTTAATTTCATTACAACTATAATCAATATACGAGCACCTGGGATGTCATTAGATCAAACCCCATTATTTGTATGAGCTGTTGGAATCACTGCCCTCCTCCTATTATTATCTTTACCTGTTCTTGCGGGTGCAATCACAATATTACTAACAGATCGAAATCTGAATACATCATTTTTTGACCCCGCAGGAGGGGGTGATCCCATCTTATATCAACACTTATTTTGATTTTTTGGTCACCCTGGAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Acheta domesticus was found to be negative for Wolbachia.
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