Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/30/2024 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | River Valley Community College |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | Lane 1: ladder Lane 2: Sample 1 AW01 Lane 3: Sample 2 AW01 Lane 4: Sample 1 RD01 Lane 5: Sample 2 RD02 Lane 6: Wolbachia DNA Lane 7: Water Lane 8: (+) Control Lane 9: (-) Control |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No banding at ~450bp on gel for 16s rRNA gene. All controls show that correct DNA was amplified for both arthropod CO1 and Wolbachia 16s rRNA genes. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
GCAAATTAACTTCATTTTCGGAGCTTGAGCTGGATAGTAGGTACCTCTTTAAGTATCTTAATTCGAACGGAACTAGGACAACCAGGTTATTTAATTGGAGATGATCAAACATATAATGTTATCGTAACTGCACATGCATTTGTCATAATTTTTTTCATGGTTATACCAATTATAATTGGTGGATTCGGAAATTGATTAGTACCCCTAATATTAGGTGCACCCGATATAGCCTTTCCTCGAATAAACAATATAAGATTTTGACTTTTACCACCCTCACTAACCCTTTTATTAACCAGAAGAATAGTCGAAAATGGTGCAGGGACAGGATGAACAGTTTATCCACCTTTATCAACAGGAATCGCCCACGCCGGAGCATCTGTTGATTTAGCCATTTTTTCATTACACTTAGCTGGAATTTCATCAATTCTGGGAGCCGTTAATTTCATTACAACTATAATCAATATACGAGCACCTGGGATGTCATTAGATCAAACCCCATTATTTGTATGAGCTGTTGGAATCACTGCCCTCCTCCTATTATTATCTTTACCTGTTCTTGCGGGTGCAATCACAATATTACTAACAGATCGAAATCTGAATACATCATTTTTTGACCCCGCAGGAGGGGGTGATCCCATCTTATATCAACACTTATTTTGATTTTTTGGTCACCCTGGAAGTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Acheta domesticus was found to be negative for Wolbachia. |
Water Boatman