DEMAASp24-5 Hymenoptera

Sample information
Picture
Location
Collection date	01/22/2024
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	collected using sweep net found in the bushes and dirt it was cold but sunny outside
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera
Methods
Extraction kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	The results were supportive of my prediction and other outside literature was also supportive of the results. Both controls in the gel electrophoresis were accurate and the results matched that of the negative control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hymenoptera was found to be negative for Wolbachia.

The Wolbachia Project Database