DEMAASp24-5 Hymenoptera

Sample information

Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University

collected using sweep net

found in the bushes and dirt

it was cold but sunny outside

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used


Wolbachia presence No
Confidence level High
Explanation of confidence level

The results were supportive of my prediction and other outside literature was also supportive of the results. Both controls in the gel electrophoresis were accurate and the results matched that of the negative control.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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