DEMAASp24-23 Coleoptera

Sample information
Picture	Photos by: Kyla W.
Location	No map location data available.
Collection date	01/22/2024
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Collection Method: Plastic jar and hands Collection Habitat: Cold, damp, dirt area Weather Conditions: ~ 50 degrees Fahrenheit, windy, & partial cloud coverage
Putative identification	Arthropoda Hexapoda Insecta Coleoptera
Methods
Extraction kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Controls were as expected signaling there was no cross contamination. Prior research supports negative Wolbachia presence in Coleoptera.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Coleoptera was found to be negative for Wolbachia.

The Wolbachia Project Database