DEMAASp24-23 Coleoptera

April 8, 2024

Sample information
Picture
Photos by: Kyla W.
Location No map location data available.
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Method: Plastic jar and hands

Collection Habitat: Cold, damp, dirt area

Weather Conditions: ~ 50 degrees Fahrenheit, windy, & partial cloud coverage
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls were as expected signaling there was no cross contamination. Prior research supports negative Wolbachia presence in Coleoptera.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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