DEMAASp24-22 Hymenoptera

April 8, 2024

Sample information
Picture
Photos by: Alfons T
Location
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • To obtain specimens for our Wolbachia project, we took a D-net to the pond on the northeast side of campus.
  • Between 1:05pm and 1:20pm on January 22, 2024, we collected our specimens. It was approximately 55 degrees and slightly windy outside.
  • Dipping the D-net into the pond, we did not find any insects, but found small fish and a tiny crustacean. We also discovered a few tiny creatures that resembled roly-poly bugs.
  • We relocated to the bank of the pond where we discovered a large mound of dirt. We agitated the mound with tweezers, causing ants to stream out.
  • We lowered our collection jar to the ground and allowed the ants to enter, catching several.
  • The area was quite swampy while we were collecting, but we did note that the dirt where the ants were collected was very dry
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit tweezers
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There is no second band in DEMAASp24-22 which indicates a lack of Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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