DEMAASp24-3 Hymenoptera

April 8, 2024

Sample information
Picture
Photos by: Craig B
Location
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

outside the science center on a rose caught with a sweep net it was 50 degrees Fahrenheit  at 1:20pm
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no  cross contamination, Every test ran came back with satisfactory results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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