DEMAASp24-16-Coleoptera

April 8, 2024

Sample information
Picture
Photos by: Ashlynn L
Location No map location data available.
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found on sidewalk by bushes near the library on campus. Use forceps to pick up and place in container.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Found on sidewalk near bushed next to lane library on campus
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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