April 8, 2024

Sample information
Picture
Photos by: Jakhari R.
Location
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Hexapoda Insecta Neuroptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
GGCATGCNNNNNNNCGATNANNNNNGATTCCAACTTCTGTACTCGAGTTGCAGAGTACAATCCGAAC TGAGATGTCTTTTAGGGATTAGCTTANGCTTGCGCACCTTGCAGCCCATTGTANACACCATTGTNNC NNNTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCCCACCTTCCTCCANCTTATCA CTGGCAGTTTCCTTANAGNACTCAGCATTACCTGATAGCAACTAAGGATGAGGGTTGCGCTCGTTGC GGGACTTAACCCANNATCTCACGACACGAGCTGACGACAGCCATGCNACACTTGNGTAANATCCGG CCGAACCGACCCTATCCCTTCGAATAGGTATGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Neuroptera was found to be negative for Wolbachia.
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