April 8, 2024

Sample information
Picture
Location No map location data available.
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Time frame: 1:05-1:25
  • Location of collection: A pond behind the parking lot
  • Environment of collection: It was in the pond near the grassy and muddy area
  • Method of collection: A D net
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level was high because I felt my insect would’ve been infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Odonata was found to be negative for Wolbachia.
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