SDDJER-S16-02 Dermaptera

April 9, 2024

Sample information
Picture
Photos by: Dejanique Pratt
Location No map location data available.
Collection date 04/09/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

We found insects under logs, in the dirt ant piles, by a damp area of water around 10:15-10:24 on January 23rd.
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I expected my insect to not be present with Wolbachia and it came back negative therefore my expectancy rate which is high is true.
Wolbachia 16S sequence
CATACCTATTCGAAGGGATAGGGTCGGTTCGGCCGGGTTTCACACAGGTGTT GCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACG AGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGA AACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGG CCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGC AAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTA CTCTGCAACTCGAGTGCATGAAG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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