Sample information |
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| Picture |
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|---|---|
| Location | No map location data available. |
| Collection date | 04/09/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | We found insects under logs, in the dirt ant piles, by a damp area of water around 10:15-10:24 on January 23rd. |
| Putative identification | Arthropoda Hexapoda Insecta Dermaptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I expected my insect to not be present with Wolbachia and it came back negative therefore my expectancy rate which is high is true. |
| Wolbachia 16S sequence |
CATACCTATTCGAAGGGATAGGGTCGGTTCGGCCGGGTTTCACACAGGTGTT GCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACG AGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGA AACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGG CCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGC AAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTA CTCTGCAACTCGAGTGCATGAAG
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Dermaptera was found to be negative for Wolbachia. |
Water Boatman