LOGADS24-RJB Hymenoptera

April 9, 2024

Sample information
Picture
Photos by: Robert B.
Location
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The insect was crawling on the leaf of a bush when we caught it. We could tell it was an ant-like insect but were unsure if it definitely was because the mandibles were not visible until we put it under a microscope. It could have been a mimic. However once we did that analysis it became clear it was definitely an ant and definitely part of the Hymenoptera order. However, I have yet to be able to identify the exact species.

Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents are used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our gel came out with the respected results. There were no obvious errors and we were careful during all steps of the process. There is little reason to believe the results are inaccurate.  The results also helped support my hypothesis, since local prevalence of my order was low, I expected that it would be negative for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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