Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/24/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | The insect was crawling on the leaf of a bush when we caught it. We could tell it was an ant-like insect but were unsure if it definitely was because the mandibles were not visible until we put it under a microscope. It could have been a mimic. However once we did that analysis it became clear it was definitely an ant and definitely part of the Hymenoptera order. However, I have yet to be able to identify the exact species. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents are used. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Our gel came out with the respected results. There were no obvious errors and we were careful during all steps of the process. There is little reason to believe the results are inaccurate. The results also helped support my hypothesis, since local prevalence of my order was low, I expected that it would be negative for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman