MACAESP24-19 Hymenoptera

April 9, 2024

Sample information
Picture
Photos by: Lauren, M.
Location
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Weather conditions: Cloudy, 64°

Location: Armstrong Campus Garden next to Campus Police Building

Collection Method: Tray Collection sifting through dirt with gloves

Habitat: Found in compost bed in moist dirt and found underneath a brick
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Based on the research of insects in the order Hymenoptera, I did not predict the insect to contain Wolbachia DNA, however, the quality of the DNA was not great as the A260/A280 value was below 1.7 and this could have affected the results. Overall results of the controls were as expected so I am highly confident that these results can be trusted.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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