Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Weather conditions: Cloudy, 64° Location: Armstrong Campus Garden next to Campus Police Building Collection Method: Tray Collection sifting through dirt with gloves Habitat: Found in compost bed in moist dirt and found underneath a brick |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Based on the research of insects in the order Hymenoptera, I did not predict the insect to contain Wolbachia DNA, however, the quality of the DNA was not great as the A260/A280 value was below 1.7 and this could have affected the results. Overall results of the controls were as expected so I am highly confident that these results can be trusted. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman