MACAESP24-19 Hymenoptera

Sample information
Picture	Photos by: Lauren, M.
Location
Collection date	01/23/2024
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Weather conditions: Cloudy, 64° Location: Armstrong Campus Garden next to Campus Police Building Collection Method: Tray Collection sifting through dirt with gloves Habitat: Found in compost bed in moist dirt and found underneath a brick
Putative identification	Arthropoda Hexapoda Insecta Hymenoptera
Methods
Extraction kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	Based on the research of insects in the order Hymenoptera, I did not predict the insect to contain Wolbachia DNA, however, the quality of the DNA was not great as the A260/A280 value was below 1.7 and this could have affected the results. Overall results of the controls were as expected so I am highly confident that these results can be trusted.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hymenoptera was found to be negative for Wolbachia.

The Wolbachia Project Database