MAC.AE.SP24-06 Hemiptera

April 9, 2024

Sample information
Picture
Photos by: Isabella D.
Location
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Besides the armstrong pond on the side closest to the greenhouse. In muddy area right beside the pond.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Insects of the order Hemiptera tend to have low numbers of insects with Wolbachia. I felt confident in my results because I did not make any mistakes during the process and I strictly followed directions during all of the testing.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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