KELAGSP24KC

April 10, 2024

Sample information
Picture
Photos by: Khiyona C
Location
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Collected at 1:45 PM
  • Behind Armstrong Georgia Southern University’s Science Center
  • Caught with a net in wet terrain with bushes and tall grass
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The gel at first looked like it was negative but later was found to be positive due to the glow of the gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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