KELAGSP-AS Coleoptera

April 10, 2024

Sample information
Picture
Photos by: Aubrie S.
Location No map location data available.
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Was caught on some bushes on campus using a sweep net.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

It is difficult to specify whether the result is positive or negative. In the gel image, a faint second band can be seen. The lab did not come back with any conclusive results, so it’s up to personal interpretation.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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