Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/24/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | headroom by Armstrong east pond, time 13:15 weather overcast, drizzle 68 degrees Fahrenheit found in golden rod grass |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | positive results were accurate with test samples |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be postive for Wolbachia. |
Water Boatman