DEMAFSP24-8 Hemiptera

April 10, 2024

Sample information
Picture
Photos by: Mark M
Location
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

headroom by Armstrong east pond, time 13:15 weather overcast, drizzle 68 degrees Fahrenheit found in golden rod grass
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

positive results were accurate with test samples
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be postive for Wolbachia.
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