April 10, 2024

Sample information
Picture
Location
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Habitat: Grasslands – wet, damp conditions​
  • Time: 1:00-1:30 p.m.​
  • Method: Sweep net​
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit none
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Very confident
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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