DEMAFsp24-22 Dermaptera

Sample information
Picture	Photos by: Ava H
Location
Collection date	01/24/2024
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Completed on 1/24/24 at 1-1:30 pm, class completed in-lab insect collection. Location: field by the greenhouse on the GSU Armstrong campus Environment/ Habitat: Terrestrial (land-based)/ damp due to rain Location: Field, looking under logs and tall grass with sweep net (collection technique) Discovery: Many bugs (ants, beetles, worms, and many unknown species) Temperature: moderately warm, more bugs were out and were easier to catch
Putative identification	Arthropoda Hexapoda Insecta Dermaptera
Methods
Extraction kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	My confidence level is high. After finishing my project viewers can see that the data controls worked. Not only that, they also lined up with my prediction/local data which states that Wolbachia will not be precent in Dermaptera.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Dermaptera was found to be negative for Wolbachia.

The Wolbachia Project Database