Sample information
Picture	Photos by: Humna H.
Location
Collection date	09/01/2023
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Scarabaeidae Cyclocephala Cyclocephala borealis
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Unknown
Confidence level	Low
Explanation of confidence level	We were unable to sequence arthropod nor Wolbachia DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary