Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/05/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2023 Students |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | No wolbachia bands on gel electrophoresis |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
Oxidus gracilis, VG2
Oniscus asellus, VG1
Tachypodoiulus niger LL2
Oniscus asellus LL1
Tachypodoiulus niger LB1