Sample information
Picture	Photos by: Khaled A.
Location
Collection date	09/05/2023
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Collected by Fall 2023 Students
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	No wolbachia bands on gel electrophoresis
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA    Download AB1
Summary	The Arthropoda was found to be negative for Wolbachia.