Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/05/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Small, black, and red insect with black markings on red abdominal region. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Cydnidae Sehirus Sehirus cinctus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My confidence level is moderate because, the gel electrophoresis results showed a faint band positive for Wolbachia. The controls worked, the negative control was negative for Wolbachia and the positive control was positive for Wolbachia, there were no signs of contamination. However, in the class results for Sanger sequencing, only one came back positive for the presence of Wolbachia gene sequence and it wasn’t my arthropod. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Sehirus cinctus was found to be postive for Wolbachia. |
Metriocnemus fuscipes YC1
Trachelipus ratzeburgii YC2
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1