Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 02/25/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | |
| Putative identification | Arthropoda |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence |
CTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Arthropoda was found to be postive for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)