Sample information

Photos by: Gabe L.
Collection date 03/15/2024
Captive / Cultivated? Wild-caught
Group River Valley Community College

Medium sized tick found sucking blood on a house cat (indoor/outdoor cat).

Putative identification Arthropoda Chelicerata Arachnida Ixodida


Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Doubled recipe for Gel in step 6.


Wolbachia presence No
Confidence level Low
Explanation of confidence level

One of our positive controls from the extraction section and our DNA from the PCR section both came out with unexpected results which makes these results questionable despite not being out of the ordinary.

The sequence quality analysis also showed very messy overlapping nucleotides indicating contamination in the results. BLAST also could not correlate the data to any known DNA sequences.

Wolbachia 16S sequence
Arthropod COI sequence Download AB1
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Ixodida was found to be negative for Wolbachia.
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