Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/15/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | River Valley Community College |
| Observations | Medium sized tick found sucking blood on a house cat (indoor/outdoor cat). |
| Putative identification | Arthropoda Chelicerata Arachnida Ixodida |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
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| Protocol notes | Doubled recipe for Gel in step 6. |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | One of our positive controls from the extraction section and our DNA from the PCR section both came out with unexpected results which makes these results questionable despite not being out of the ordinary. The sequence quality analysis also showed very messy overlapping nucleotides indicating contamination in the results. BLAST also could not correlate the data to any known DNA sequences. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
CCCNGNAGNNATNGNNCCNNCATACACTTCATTTCGACGATTTNATGGTTGCTGTTTCTTTTAATNACGNACGAGTGCGTGACNGACACAGTACTNCCCCTTTCTTATACAGGGGCCTGCACGGGNAANNTGGTNCTNCACACCACTACTCNCNTCCTNGNCGTGGTGAANAAGCAAAGACGGANTAGGTNACCCCTCAGGTTATNCCGTGGTGGGGGGCTNTCGGNCCACANAAANCACAACAGATAGGCGNCCTGGGAGAGCCNCCGTCCACANTGGTACTGAGACGCTGGNACTANACCNNNTAAAGGNCTCCTAGTGGAATATTGAGGANTGNGCGATCNACTGAGGCCGANACCCGGCGGACGNTGATGGGCTNTGGGGTGTGAATTTNTTTCATTCANGAATAAGCGAAAGTAACGATACTTNCAGAAGAAGCTGNCGGTACCTTCGNGNTACCTTACGNGGAAGTACGTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Ixodida was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)