Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/14/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | found under dead tree trunk, singular found |
| Putative identification | Arthropoda Myriapoda Diplopoda Julida Julidae Tachypodoiulus Tachypodoiulus niger |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | No Wolbachia sequence was found. So there could have been a mistake in the preparation of the DNA sequence / PCR or there is really no sign of a Wolbachia sequence. Therefore is the result unknown. |
| Wolbachia 16S sequence |
-
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
-
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Brachycybe rosea
Dermacentor variabilis
Hypnorna sp.
Dermacentor variabilis
Dermacentor variabilis