Cylindroiulus punctatus XJ

Sample information
Picture	Photos by: Xenia J.
Location
Collection date	03/14/2024
Captive / Cultivated?	Wild-caught
Group	KantiWil
Observations	Caught under the tree bark of a log in a pile of dead wood at the entrance of a forest.
Putative identification	Arthropoda Myriapoda Diplopoda Julida Julidae Cylindroiulus Cylindroiulus punctatus
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	Low
Explanation of confidence level	There is a band at approximately 438 base pairs. However, there is no second band at 708 base pairs, which signifies that there might have been some kind of mistake in the process. The result might be correct, if the primers simply did not catch onto the cytochromoxidase gene. (Position XJ2)
Wolbachia 16S sequence	Download FASTA Download AB1 TGGGTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGYCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGKGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGT BLAST at The Wolbachia Project BLAST at NCBI
Arthropod COI sequence
Summary	The Cylindroiulus punctatus was found to be postive for Wolbachia.

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