Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/27/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations |
J3 through J6 tested for Wolbachia gene 16s, J3-Wasp, J4-large cricket, J5-Fly, J6-Small cricket, J7 through J10 tested for Arthropod gene Co1, J7-wasp, J8-Large cricket, J9-Fly, J10-Small cricket, arrows indicate positive gene, J5-tested pos. for 16s, J7+J9 tested pos. For Co1 |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Polleniidae Pollenia Pollenia pediculata |
Methods |
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| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | LB |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence |
jaime_samp5_WO_Wolbachia_pipientis_strain GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCC
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
jaime_samp5_CO1_7C_Pollenia_pediculata_voucher TCTTTAAGAATATTAGTTCGAGCAGAATTAGGACATCCTGGAGCATTAATCGGTGATGATCAAATTTATAATGTAATTGTAACAGCCCATGCCTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTTCCTTTAATATTAGGAGCACCTGATATAGCATTCCCTCGAATGAATAATATAAGTTTTTGACTCTTACCTCCTGCACTTACATTATTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCACCTCTATCTTCTAATATTGCTCATGGAGGGGCTTCTGTTGATTTAGCTATTTTTTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTAATTAATATACGATCTACAGGTATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTTCTTTTATCCTTACCTGTATTAGCAGGAGCTATCACTATATTATTAACAGATCGAAATTTAAATACTTCCTTTTTTGATCCTGCAGGAGGAGGAGACCCAATTTTATATCAACA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Pollenia pediculata was found to be postive for Wolbachia. |
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