Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Talladega College |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Lampyridae Photinus Photinus pyralis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | PCR reaction ran with positive and negative controls as well as Wolbachia positive fruit flies. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
GGCAATTTTNTCTTTGGGGCATGAGAGGAATACTAGGAACTTCATTCAGATTATTAATTCGTGCAGAATTAGGAATTCCGGGATCCTTAATTGGAAATGATCATATTTTTAATGTAATTGTTACTAGACACGCCTTCATTATAATTTTTTTTATAGTAATGCCTATCATAATTGGGGGCTTCGGGAATTGGTTAGTTCCCTTAATACTCGGGGCTCCTGATATAGCTTTTCCTCGAATAAACAATATAAGATTTTGATTATTACCCCCATCCCTATCTCTCTTAATTATAAGAAGAATAATTGAAAGAGGGGCAGGAACTGGCTGAACTGTCTACCCTCCCCTGTCAGCTAATATTGCTCACAGAGGTCCTTCAGTAGATTTAGCAATTTTTAGACTTCATCTTGCTGGGGTATCCTCAATTCTAGGTGCAGTAAACTTTATTTCAACTATTATTAATATACGGCCAAATAGAATAATATTAGATCAAATACCCTTATTTGTTTGAGCAGTTCTAATTACCGCAATTCTTTTATTATTATCATTACCCGTATTAGCAGGTGCTATTACTATACTCTTAACAGATCGAAATTTAAATTCTTCTTTTTTTGATCCGGCAGAAGGAGGAGATCCAGTTTTATACCAACACTTATTTTGATTTTTGGCACGCCAANNNNNNNAAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Photinus pyralis was found to be negative for Wolbachia. |
Brachycybe rosea
Dermacentor variabilis
Hypnorna sp.
Dermacentor variabilis
Dermacentor variabilis