Sample information
Picture	Photos by: Kyla P.
Location
Collection date	05/14/2024
Captive / Cultivated?	Wild-caught
Group	California Academy of Mathematics and Science
Observations	I found the arthropod in the brush, mixed with flowers and weeds in between multiple buildings.
Putative identification	Arthropoda Hexapoda Insecta Diptera Anthomyiidae
Methods
Extraction kit	pcr
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Fast Blast
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Anthomyiidae was found to be negative for Wolbachia.