GRP12 – House Fly/Musca domestica

May 23, 2024

Sample information
Picture
Photos by: Natalie A., Juan C., Analis G., Xenia M.
Location
Collection date 04/23/2024
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

This fly was caught in a household as it flew in and out of a window.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain UView
Gel images
Protocol notes

Ran gel for 60 minutes at 115 V and 60amps and visualized under UV light.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Our confidence level is high that the housefly does not have Wolbachia because there are no bands at all in lane 10 (housefly), therefore there is no visible presence of Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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